Programma

The Course aims to provide students with basic knowledge relating to biochemistry and cellular metabolism. Carbohydrates, Lipids, Amino acids, proteins: structure of proteins and examples of structure-function relationships. Enzymes and enzymatic catalysis: Definition of active site and substrate specificity; the Michaelis-Menten equation, enzyme inhibition. Introduction to aerobic and anaerobic metabolism. Metabolism of carbohydrates, lipids and nitrogen compounds. Energy production in mitochondria.
The Course also aims to provide basic knowledge of molecular biology. Purine and pyrimidine nucleotides; structure of biological macromolecules; structure of prokaryotic and eukaryotic chromosomes; Replication, Transcription; Translation; Recombinant DNA and Genetically modified organisms.
Laboratory activities related to recombinant DNA technology.

Testi Adottati

Recommended textbooks:
Chimica, biochimica e biologia applicata. Stefani M., Taddei N. ed. Zanichelli, 2015.
Elementi di biologia e genetica. Sadana D., Hillis DM., Heller Hc, Berenbaum Mr. Ed. Zanichelli, 2014.

Lesson slides will be provided.
The professor Cervelli receives every day from 10.00 to 11.00 by appointment via e-mail: manuela.cervelli@uniroma3.it

Bibliografia Di Riferimento

Chimica, biochimica e biologia applicata. Stefani M., Taddei N. ed. Zanichelli, 2015. Elementi di biologia e genetica. Sadana D., Hillis DM., Heller Hc, Berenbaum Mr. Ed. Zanichelli, 2014.

Modalità Erogazione

The course will take place with lectures and training in didactic laboratories.

Modalità Frequenza

The course will take place with lectures and training in didactic laboratories.

Modalità Valutazione

The written test will focus on a brief description of 2-4 topics held studied the course. The score will take into account both the knowledge of the subject and the ability to present it synthetically. Mid-term tests will be carried out with the attribution of a score effective for the final vote.

Programma

PLANT METABOLISM:
Water balance and mineral nutrition of plants; Photosynthetic metabolism; Nitrogen assimilation; Photoreceptors; plant hormones.
INTRODUCTION TO PLANT METABOLISM:
Definition of transgenic plant; Methods of plant transformation; Applications of Plant Biotecnology.

Laboratory activities:
-Protein extraction from plant tissues;
-Application of an assay ('Bradford') for protein quantification;
-Catalytic activity assays for plant enzymes;
-Genotyping of transgenic plants through DNA extraction, PCR and electrophoretic analysis of the PCR products;
-Discussion on the obtained results.

Testi Adottati

Recommended textbooks:
Elementi di Fisiologia Vegetale (N. Rascio), EdiSES, Edizione III, 2021
Lecture notes will be provided

Modalità Erogazione

The course will take place through classroom lectures and laboratory activities. Lectures are organized by means of power point presentations that are made available to the students. The laboratory activities are organized in such a way to allow each student to beneficiate a single experimental position (or in pairs) and to actively participate to the activities. Before each laboratory activity, students are adequately informed and trained about the risks inherent in the exercise and provided with safety data sheets for the materials used and individual protection devices. Students complete and countersign a declaration of training / information / training regarding the aforementioned risks. Each laboratory activity is accompanied by a training course carried out by means of a printed informative material which is provided to each student and which contains questions of verification of comprehension, ability to calculate, ability to apply to problems associated with the exercise.

Modalità Frequenza

Student presence during the lessons is mandatory. Remote teaching is organized only for the categories of students authorized by the University.

Modalità Valutazione

The written test will focus on a brief description of 2-5 topics held during the course. The score will take into account both the knowledge of the subject and the ability to present it synthetically. Mid-term written tests will be carried out (a brief description of4 topics held during the course), with the attribution of a score effective for the final vote.

Programma

Protein structure and examples of structure-function relationships. Enzymes and enzymatic catalysis: definition of active site and substrate specificity; the Michaelis-Menten equation, enzyme inhibition. Introduction to aerobic and anaerobic metabolism. Metabolism of carbohydrates, lipids, and nitrogen compounds.

Testi Adottati

Chimica, biochimica e biologia applicata. Stefani M., Taddei N. ed. Zanichelli, 2015.
Elementi di biologia e genetica. Sadana D., Hillis DM., Heller Hc, Berenbaum Mr. Ed. Zanichelli, 2014.

Modalità Frequenza

Non-mandatory attendance

Modalità Valutazione

Written exam