Programma

1. BASIC TECHNOLOGIES OF GENE ANALYSIS; RESTRICTION ENZYMES; ELECTROPHORETIC ANALYSIS. 2. VECTORS; CLONING. 3. PCR; REAL TIME PCR; PCR APPLICATIONS. 4. GENETIC VARIABILITY APPLIED TO FORENSIC SCIENCE 5. GENE IDENTIFICATION; GENOMIC LIBRERIES AND cDNA LIBRERIES; YEAST TWO HYBRID SYSTEM 6. MUTAGENESIS. 7. RECOMBINANT PROTEIN PRODUCTION AND PURIFICATION. 8. FROM SANGER SEQUENCING TO NEXT-GENERATION SEQUENCING. 9. POST-GENOMICS ANALYSIS; TRANSCRIPTOMIC ANALYSIS; REPORTER GENES. 10. PROTEOMIC ANALYSIS. 11. PRODUCTION AND ANALYSIS OF TRANSGENIC PLANTS. 12. GENETIC MODIFICATION OF ANIMAL CELLS; TRANSGENIC ANIMALS CONSTRUCTION. 13. GENE TRAPPING; GENE TARGETING. 14. ANIMAL CLONING; GENE THERAPY. 15. THE CRISPR-CAS SYSTEM: EMERGING TECHNOLOGY AND APPLICATION AND APPLICATIONS.

Testi Adottati

Recommended textbooks:
1. METODOLOGIE BIOCHIMICHE STRUMENTI E TECNICHE PER IL LABORATORIO DEL NUOVO MILLENNIO, MACCARRONE M., E BIOMOLECOLARI, CASA EDITRICE ZANICHELLI
2. DAI GENI AI GENOMI, JEREMY W. DALE, MALCOLM VON SCHANTZ, NICK PLANT, A CURA DI: E. GINELLI, CASA EDITRICE EDISES
3. ANALISI DEI GENI E GENOMI, REECE, EDISES.
4. TECNICHE E METODI PER LA BIOLOGIA MOLECOLARE, AMALDI F., BENEDETTI P., PESOLE G., PLEVANI P., CASA EDITRICE AMBROSIANA.

Lecture notes and calculation exercises will be provided.
Non-attending students are invited to request information on the program, teaching materials and how to evaluate the profit.
The professor receives every day from 10.00 to 11.00 by appointment via e-mail: manuela.cervelli@uniroma3.it

Bibliografia Di Riferimento

The Professor will provide scientific articles concerning the topics discussed.

Modalità Erogazione

The course will take place with lectures and training in didactic laboratories.

Modalità Frequenza

The course will take place with lectures and training in didactic laboratories.

Modalità Valutazione

Oral examination. The test is in an interview of about 30 minutes during the period the student has to answer a series of questions (5-6) including most of the topics covered during the course. Each vote takes into account the acquired knowledge (50%), the student's ability to integrate different concepts and concepts and insert them correctly in contextual problems (30%), and the ability to offer a clear language, correct to the contents (20%).

Programma

THE COURSE OF MOLECULAR BIOLOGY IS PART OF THE TEACHING ACTIVITIES OF THE DEGREE COURSE IN LIFE SCIENCES. THE COURSE THROUGH FRONTAL LESSONS AND LABORATORY TRAINING AIMS TO PROVIDE THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. TOOLS WILL BE GIVEN TO UNDERSTAND THE MAIN MOLECULAR BIOLOGY STRATEGIES AND TECHNIQUES UTILIZED TO STUDY GENES AND GENOMES. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE STUDENTS WILL GET ACQUAINTED WITH THEORETICAL KNOWLEDGES AND METHODOLOGICAL SKILLS THAT WILL ENABLE THEM TO APPLY THE CORRECT STUDY TO SOLVE SPECIFIC PROBLEMS IN THE FIELD OF MOLECULAR BIOLOGY.
THE COURSE CONCERNS THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEUDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE TEACHING PROGRAM IS ORGANIZED AS FOLLOWS: GENOME ANATOMY, TRANSCRIPTOMES AND PROTEOMES; GENOME ACCESSIBILITY; TRANSCRIPTION INITIATION COMPLEX; RNA SYNTHESES AND MATURATION; PROTEOME SYNTHESES AND MATURATION; GENOME SEQUENCING; GENOME REPLICATION; DNA REPAIR SYSTEMS; GENETIC RECOMBINATION; GENETIC ENGINEERING AND DNA RECOMBINANT TECHNIQUES.

Testi Adottati

BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI
BIOLOGIA MOLECOLARE, CAPRANICO ET AL, II EDIZIONE, CASA EDITRICE EDISES
FONDAMENTI DI BIOLOGIA MOLECOLARE, L.A. ALLISON, SECONDA EDIZIONE ITALIANA, CASA EDITRICE ZANICHELLI
The professor receives every day from 8.00 to 9.00 by appointment via e-mail: giovanni.antonini@uniroma3.it

Modalità Erogazione

Attendance is strongly recommended

Modalità Frequenza

Attendance at lessons is strongly recommended

Modalità Valutazione

The exam will consist of an oral test on the topics presented in the program

Programma

Basic molecular techniques for the manipulation of nucleic acids. Students will learn how to use pipettes and other common laboratory instruments, and will tackle the various experimental steps aimed at cloning a DNA fragment. In particular, they will explore the use of restriction/modification enzymes, polymerase chain reaction (PCR), and its applications. Finally, they will conduct electrophoretic analysis on agarose gel for the separation of DNA fragments produced by PCR.

Testi Adottati

REFERENCE TEXTBOOK:
BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI.
FURTHER SUGGESTED TEXTBOOKS: 1) GENOMI, BROWN, QUARTA EDIZIONE, CASA EDITRICE EDISES; 2) IL GENE, LEWIN, TERZA EDIZIONE COMPATTA, ZANICHELLI.
1. ANALISI DEI GENI E GENOMI, REECE, EDISES.
2. DNA RICOMBINANTE, J.D.WATSON, A.A. CAUDY, R.M. MYERS E J.A. WITKOWSKI, ZANICHELLI.

Bibliografia Di Riferimento

REFERENCE TEXTBOOK: BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI. FURTHER SUGGESTED TEXTBOOKS: 1) GENOMI, BROWN, QUARTA EDIZIONE, CASA EDITRICE EDISES; 2) IL GENE, LEWIN, TERZA EDIZIONE COMPATTA, ZANICHELLI. 1. ANALISI DEI GENI E GENOMI, REECE, EDISES. 2. DNA RICOMBINANTE, J.D.WATSON, A.A. CAUDY, R.M. MYERS E J.A. WITKOWSKI, ZANICHELLI.

Modalità Frequenza

The course will be held with frontal lessons

Modalità Valutazione

Oral examination. The oral exam consists in an interview of about 10 minutes and will focus on the topics covered during the laboratory experience. The day of the examination a written report on the laboratory experience has to be presented and discussed, demonstrating to have understood the theoretical and practical principles of the techniques carried out during the laboratory experience. Furthermore, the ability to critically discuss the obtained results is required.