|Sezione:||Scienze e Tecnologie Biomediche|
|Gruppi e laboratori di ricerca:||MICROBIAL BIOTECNOLOGY (BIOTECNOLOGIE DEI MICRORGANISMI)|
Biotecnologie dei microrganismi
Viale Marconi 446
2002_Specialization degree in Microbiology and Virology (Medical School, post-graduation degree);
1997_PhD in Public Health (specialism in Microbiology);
1993_ Master degree in Biological Sciences with full marks and honours (110/110 cum laude);
All Livia Leoni’s degrees are from the University of Rome, “La Sapienza”, Italy.
Professional experience and granted fellowships
2012- today permanent position as Associate Professor in Microbial Biotechnology at the Dept. of Biology (now Dept. of Science) of the University of Roma Tre;
2000-2012. Staff scientist (Tecnico Laureato) at the Dept. of Biology of the University of Roma Tre (now Dept. of Science);
1997-2000. Post-doc position. Institute of Microbiology, School of Medicine of the University “La Sapienza” Rome;
1997-2000. Fellowship from the post-graduate school of Microbiology and Virology. University of Roma, “La Sapienza”:
1996. FEMS fellowship. Centro Nacional de Biotecnologia del’Università Autonoma di Madrid, Spagna. (supervisors Profs. Victor de Lorenzo and Paolo Visca);
1993-1997. PhD fellowship. Institute of Microbiology, School of Medicine of the University “La Sapienza” Rome (Supervisors, Profs. Paolo Visca and Nicola Orsi).
Memberships and Appointments
Coordinator of the Bachelor Degree in "Enogastronomic Science and Culture"; Member of the Scientific Directory board of the Doctoral School in Biomedical Sciences and Technologies of the University Roma Tre; Academic Editor for PLOS ONE.
Member of the following Scientific societies: SIMGBM (Italian General Microbiology and Microbial Biotechnology Society), FEMS (Federation of European Microbiology Societies) and ASM (American Society for Microbiology);
Reviewer for: Annals of Microbiology, Applied and Environmental Microbiology, Applied Microbiology, BMC Genomics, BMC Microbiology, Current Microbiology, FEMS Immunology and Medical Microbiology, FEMS Microbiology Letters, Future Medicine, International Journal of Antimicrobial Agents International Journal of Molecular Sciences Journal of Global Antimicrobial Letters of Applied Microbiology Microbiology, PLoS One, PLoS Pathogen, The World Journal of Microbiology and Biotechnology; (alphabetic order).
2013-2015, director of the Post-Graduate School in Industrial Health. Dept. of Science, University Roma Tre;
2013-2010, member of the SIMGBM Scientific Directory Board (Italian General Microbiology and Microbial Biotechnology Society);
2011 and 2013, member of the Scientific Committee of 29° and 30° SIMGBM International conferences;
2011. European Community Expert for the evaluation of Marie Curie Individual fellowships calls FP7-PEOPLE-IEF-IIF-IOF-HEALTH panel;
Attività di ricerca
Since 1993 Livia Leoni has been studying bacteria belonging to the Pseudomonas genus, with special focus on global regulatory networks governing leading processes such as social behaviour (quorum sensing), pathogenesis, degradation of toxic compounds (bioremediation), carbon catabolite repression. Her experimental approach spans from traditional microbiology and genetics to molecular biology, biochemistry and synthetic biology.
The present research of Livia Leoni is mainly focused upon Pseudomonas aeruginosa as model organism and it is organized in the following research lines:
-understand bacterial pathogenesis and biofilm formation;
-develop high-throughput screening systems for the identification of novel compounds targeting bacterial biofilm and virulence;
-screening of libraries of FDA approved drugs for the repurposing of “old” drugs for novel anti-virulence and anti-biofilm therapies.
Coordination of Research projects
Multicentre Research projects in which Livia Leoni has been Coordinator (CO) or Responsible of Research Unit (RU) are listed below, all granted by the Italian Cystic Fibrosis Research Foundation (http://www.fibrosicisticaricerca.it/).
2013-2015 “Anti-virulence therapy against Pseudomonas aeruginosa: identification of anti-biofilm drugs and development of inhalable Niclosamide and Flucytosine formulations (CO, RU). FFC #10/2013
2011-2012 “Identification and characterization of novel drugs suppressing Pseudomonas aeruginosa virulence in chronic infection” (CO, RU). FFC #13/2011
2010-2012 “Non-conventional strategies against Pseudomonas aeruginosa infection: interference with iron homeostasis and quorum sensing” (RU). FFC #14/2010
2008-2010 "Development and validation of a novel screening system for the identification of Pseudomonas aeruginosa virulence inhibitors” (CO, RU). FFC #8/2008
2007-2009 “Iron uptake and quorum sensing in Pseudomonas aeruginosa virulence” (RU). FFC #10/2007
List of Publications (2013-2015)
- Scaccabarozzi L, Leoni L, Ballarini E, Barberio A, Locatelli B, Casula A, Bronzo V, Pisoni G, Jousson O, Morandi S, Rapetti L, García-Fernández A, Moroni P. Pseudomonas aeruginosa in dairy goats: genotypic and phenotypic comparison of intramammary and environmental isolates (2015). PLOS ONE. 10: e0142973
- Fernicola S, Paiardini A, Giardina G, Rampioni G, Leoni L, Cutruzzolà F, Rinaldo S. In Silico Discovery and In Vitro Validation of Catechol-Containing Sulfonohydrazide Compounds as Potent Inhibitors of the Diguanylate Cyclase PleD. J Bacteriol. 2015 Sep 28;198(1):147-56.
- Fernicola S, Torquati I, Paiardini A, Giardina G, Rampioni G, Messina M, Leoni L, Del Bello F, Petrelli R, Rinaldo S, Cappellacci L, Cutruzzolà F. Synthesis of Triazole-Linked Analogues of c-di-GMP and Their Interactions with Diguanylate Cyclase. J Med Chem. 2015 Oct 22;58(20):8269-84.
- Costabile G, d’Angelo I, Rampioni G, Bondì R, Pompili B, Ascenzioni F, Mitidieri E., d’Emmanuele di Villa Bianca R, Sorrentino R, Miro A, Quaglia F, Imperi F, Leoni L, Ungaro F. (2015) Repositioning niclosamide for anti-virulence therapy of Pseudomonas aeruginosa lung infections: nanocrystal-embedded dry powders for inhalation suspension. Mol Pharm. 12:2604-17
- Bondí R, Messina M, De Fino I, Bragonzi A, Rampioni G, Leoni L*. (2014) Affecting Pseudomonas aeruginosa phenotypic plasticity by quorum sensing dysregulation hampers pathogenicity in murine chronic lung infection. PLOS ONE. 24; 9:e112105
- Massai F, Rampioni G, Micolonghi C, Messina M, Zennaro E, Ascenzi P, Leoni L*. (2014) Styrene is sensed by the N-terminal PAS sensor domain of StyS, a double sensor kinase from the styrene-degrading bacterium Pseudomonas fluorescens Ann Microbiol. Published ahead of print. DOI 10.1007/s13213-014-0931-y.
- Mavelli F, Rampioni G, Damiano L, Messina M, Leoni L, Stano P. Molecular communication technology: general considerations on the use of synthetic cells and some hints from in silico modelling. Communications in Computer and Information Science (2014) 445:169-189.
- Rampioni G, Mavelli F, Damiano L, D’Angelo F, Messina M, Leoni L, Stano P. (2014) A synthetic biology approach to bio-chem-ICT: first moves towards chemical communication between synthetic and natural cells. Nat Comput. Natural Computing 13: 333-349.
- Imperi F, Leoni L, Visca P. (2014) Antivirulence activity of azithromycin in Pseudomonas aeruginosa. Frontiers of Microbiology. 5: 178.
- Rampioni G, Leoni L, Williams P. (2014) The art of war against bacteria: a deception strategy targeting quorum sensing communication systems. Bioorg Chem. Bioorg Chem. epub ahed of print. doi: 10.1016/j.bioorg.2014.04.005.
- Rampioni G, Damiano L, Messina M, D'Angelo F, Leoni L, Stano, P (2013) Chemical communication between synthetic and natural cells: a possible experimental design. Electron Proc Theor Comput Sci 130:14-26.
- Longo F, Rampioni G, Bondì R, Imperi F, Fimia GM, Visca P, Zennaro E, Leoni L*. (2013) A new transcriptional repressor of the Pseudomonas aeruginosa quorum sensing receptor gene lasR. PLoS ONE 8(7): e69554.
- Imperi F, Massai F, Facchini M, Frangipani E, Visaggio D, Leoni L, Bragonzi A, Visca P. (2013) Repurposing the antimycotic drug flucytosine for suppression of Pseudomonas aeruginosa Proc Nat Acad Sci USA. 110: 7458-63.
- Imperi F, Massai F, Ramachandran Pillai C, Longo F, Zennaro E, Rampioni G, Visca P, Leoni L*. (2013) New Life for an Old Drug: the Anthelmintic Drug Niclosamide Inhibits Pseudomonas aeruginosa Quorum Sensing. Antimicrob Agents Chemother. 57: 996-1005.
Attività di insegnamento
Corso di Biotecnologie dei Microrganismi LM in Biologia
Corso di Biotecnologie delle Fermentazioni LT in Scienze e Culture Enogastronomiche